TM6SF2 Antibody - C-terminal region
Artikelnummer:
ASB-ARP44400_P050
- Bilder (6)
| Artikelname: | TM6SF2 Antibody - C-terminal region |
| Artikelnummer: | ASB-ARP44400_P050 |
| Hersteller Artikelnummer: | ARP44400_P050 |
| Alternativnummer: | ASB-ARP44400_P050-25UL,ASB-ARP44400_P050-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | WB |
| Spezies Reaktivität: | Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat |
| Immunogen: | The immunogen is a synthetic peptide directed towards the C-terminal region of Human TM6SF2 |
| The function of this protein remains unknown. |
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Host: Rabbit Target Name: TM6SF2 Sample Type: 293T Whole cell lysates Antibody Dilution: 1.0ug/ml |
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Host: Rabbit Target: TM6SF2 Positive control (+): HepG2 (HG) Negative control (-): MCF7 (N10) Antibody concentration: 1ug/ml |
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Host: Rabbit Target Name: TM6SF2 Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0ug/ml |
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25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 5 ug/mL of the antibody was used in this experiment. Several isoforms contain the peptide sequence including ~150 kDa and smaller isoforms from 23 to 53 kDa. |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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TM6SF2 Antibody - C-terminal region (ARP44400_P050) in HepG2, MCF7 using Western Blot |
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