TGFB2 Antibody - middle region

Produktübersicht

• Artikelname: TGFB2 Antibody - middle region
• Artikelnummer: ASB-ARP45446_P050
• Hersteller Artikelnummer: ARP45446_P050
• Alternativnummer: ASB-ARP45446_P050-25UL,ASB-ARP45446_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: WB
• Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TGFB2
• Alternative Synonym: LDS4, G-TSF, TGF-beta2

Produktbeschreibung

This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types by transducing their signal through combinations of transmembrane type I and type II receptors (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins. Disruption of the TGFB/SMAD pathway has been implicated in a variety of human cancers. The encoded protein is secreted and has suppressive effects of interleukin-2 dependent T-cell growth. Translocation t(1,7)(q41,p21) between this gene and HDAC9 is associated with Peters anomaly, a congenital defect of the anterior chamber of the eye. The knockout mice lacking this gene show perinatal mortality and a wide range of developmental, including cardiac, defects. Alternatively spliced transcript variants encoding different isoforms have been identified.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 46kDa
• NCBI: 7042
• UniProt: P61812
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

WB Suggested Anti-TGFB2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: NCI-H226 cell lysateThere is BioGPS gene expression data showing that TGFB2 is expressed in NCIH226

Host: Rabbit
Target Name: TGFB2
Sample Tissue: Mouse Small Intestine
Antibody Dilution: 1ug/ml

Host: Mouse
Target Name: TGFB2
Sample Tissue: Mouse Small Intestine
Antibody Dilution: 1ug/ml

Host: Rabbit
Target: TGFB2
Positive control (+): THP-1 (N30)
Negative control (-): U937 (N31)
Antibody concentration: 3ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

TGFB2 antibody - middle region (ARP45446_P050) in Human NCI-H226 using Western Blot