The immunogen is a synthetic peptide directed towards the C terminal region of human ATP5B
Alternative Synonym:
ATP5B, ATPMB, ATPSB, HEL-S-271
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
25 ug of the indicated Mouse whole tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. The protein may be modified by phosphorylation.
Sample Type: NT2 cellsRed: AntibodyBlue: DAPIPrimary Dilution: 1ug/50ul antibodySecondary Antibody: Alexa goat anti-rabbit 594Image Submitted by: Yuzhi Chen, University of Arkansas for Medical Sciences
ATP5B antibody - C-terminal region (ARP48186_T100) validated by WB using HepG2 cell lysate at 1.25ug/ml.
ATP5B antibody - C-terminal region (ARP48186_T100) validated by WB using 1. Human NT-2 cells (60ug) 2. and 3. mouse brain extracts (80ug) 4. rat brain extract (80ug) at 2ug/ml.
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