NSUN2 Antibody - C-terminal region

Produktübersicht

• Artikelname: NSUN2 Antibody - C-terminal region
• Artikelnummer: ASB-ARP48811_P050
• Hersteller Artikelnummer: ARP48811_P050
• Alternativnummer: ASB-ARP48811_P050-25UL,ASB-ARP48811_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: WB
• Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NSUN2
• Alternative Synonym: MISU, MRT5, SAKI, TRM4

Produktbeschreibung

Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).[supplied by OMIM].

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 86 kDa
• NCBI: 54888
• UniProt: Q08J23
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. An 85 kDa isoform also contains the peptide sequence. Protein may be modified by phosphorylation.

WB Suggested Anti-NSUN2 Antibody Titration: 0.2-1 ug/ml
Positive Control: Hela cell lysate

Host: Rabbit
Target: NSUN2
Positive control (+): HeLa Cell Lysate (HL)
Negative control (-): Human Placenta (PL)
Antibody concentration: 1ug/ml

NSUN2 antibody - C-terminal region (ARP48811_P050) in Human HeLa using Western Blot