DPH1 Antibody - N-terminal region

Produktübersicht

• Artikelname: DPH1 Antibody - N-terminal region
• Artikelnummer: ASB-ARP51955_P050
• Hersteller Artikelnummer: ARP51955_P050
• Alternativnummer: ASB-ARP51955_P050-25UL,ASB-ARP51955_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: IHC, WB
• Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DPH1
• Alternative Synonym: DPH2L, OVCA1, DEDSSH, DPH2L1

Produktbeschreibung

Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 49kDa
• NCBI: 1801
• UniProt: Q9BZG8
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

Rabbit Anti-DPH1 Antibody
Catalog Number: ARP51955_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-DPH1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: RPMI 8226 cell lysate

Host: Rabbit
Target: DPH1
Positive control (+): Human Placenta (PL)
Negative control (-): MCF7 Cell Lysate (N10)
Antibody concentration: 0.5ug/ml

DPH1 antibody - N-terminal region (ARP51955_P050) in Human RPMI 8226 using Western Blot