FTH1 Antibody - middle region

Artikelnummer: ASB-ARP54620_P050
Artikelname: FTH1 Antibody - middle region
Artikelnummer: ASB-ARP54620_P050
Hersteller Artikelnummer: ARP54620_P050
Alternativnummer: ASB-ARP54620_P050-25UL,ASB-ARP54620_P050-100UL
Hersteller: Aviva
Wirt: Rabbit
Kategorie: Proteine/Peptide
Applikation: IHC, WB
Spezies Reaktivität: Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep
Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FTH1
Alternative Synonym: FHC, FTH, HFE5, PLIF, FTHL6, PIG15
FTH1 is the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Klonalität: Polyclonal
Konzentration: 0.5 mg/ml
Molekulargewicht: 21 kDa
NCBI: 2495
UniProt: P02794
Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Kidney
WB Suggested Anti-FTH1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate
Host: Rabbit
Target Name: FTH1
Sample Type: Hela
Antibody Dilution: 1.0ug/mlFTH1 is supported by BioGPS gene expression data to be expressed in HeLa
Host: Rabbit
Target Name: FTH1
Sample Type: Jurkat
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5ug/ml
Lysate Quantity: 25ug/lane/lane
Gel Concentration: 0.12
Host: Rabbit
Target Name: FTH1
Sample Tissue: Human Ovary Tumor
Antibody Dilution: 1ug/ml
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
FTH1 antibody - middle region (ARP54620_P050) in Human Jurkat using Western Blot