GCLM Antibody - middle region

Produktübersicht

• Artikelname: GCLM Antibody - middle region
• Artikelnummer: ASB-ARP54624_P050
• Hersteller Artikelnummer: ARP54624_P050
• Alternativnummer: ASB-ARP54624_P050-25UL,ASB-ARP54624_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: IHC, WB
• Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GCLM
• Alternative Synonym: GLCLR

Produktbeschreibung

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 31kDa
• NCBI: 2730
• UniProt: P48507
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

Anti-GCLM antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. GCLM Antibody ARP54624_P050 concentration 5 ug/ml.

WB Suggested Anti-GCLM Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: MCF7 cell lysate

Lanes:
1: 40ug mouse heart lysate, 2: 40ug mouse skeletal muscle lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:10000
Gene Name:
GCLM
Submitted by:
Anonymous

Host: Rabbit
Target: GCLM
Positive control (+): HepG2 (HG)
Negative control (-): Human brain (BR)
Antibody concentration: 1ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

Host: Rabbit
Target Name: GCLM
Sample Tissue: Human A549 Whole Cell
Antibody Dilution: 1ug/ml

GCLM antibody - middle region (ARP54624_P050) in Human MCF7 using Western Blot