GRN Antibody - middle region

Produktübersicht

• Artikelname: GRN Antibody - middle region
• Artikelnummer: ASB-ARP54642_P050
• Hersteller Artikelnummer: ARP54642_P050
• Alternativnummer: ASB-ARP54642_P050-25UL,ASB-ARP54642_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: WB
• Spezies Reaktivität: Equine, Human
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human GRN
• Alternative Synonym: GEP, GP88, PEPI, PGRN, CLN11, PCDGF

Produktbeschreibung

Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 64 kDa
• NCBI: 2896
• UniProt: P28799-2
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

Host: Rabbit
Target Name: GRN
Sample Type: 721_B Whole Cell lysates
Antibody Dilution: 1.0ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Canonical 62 kDa isoform is identified, and a second isoform of 52 kDa is also present in some samples.

Immunoprecipitation was performed on concentrated culture media from Brefeldin A treated HEK293T cells using 1.0 ig of the antibody pre-coupled to either protein G or protein A magnetic beads. Samples were washed and processed for immunoblot with an alternate GRN antibody at 1/1000. The Ponceau stained transfers of each blot are shown. SM=10% starting material, UB=10% unbound fraction, IP=immunoprecipitate. Data provided courtesty of the Ycharos Antibody Characterization through Open Science group. Additional data can be found at https://ycharos.com/data/.

50 ug of HEK293T WT and GRN KO lysates were subjected to SDS-PAGE followed by immunoblotting with an antibody dilution of 1/5000. The second image shows Ponceau S staining of the transferred blot as a loading control experiment. Data provided courtesty of the Ycharos Antibody Characterization through Open Science group. Additional data can be found at https://ycharos.com/data/.

Host: Rabbit
Target Name: GRN
Sample Tissue: Human 786-0 Whole Cell
Antibody Dilution: 3ug/ml

GRN Antibody (ARP54642_P050) in Human 721_B Whole Cell using Western Blot