CPEB3 Antibody - middle region
Artikelnummer:
ASB-ARP55103_P050
- Bilder (5)
| Artikelname: | CPEB3 Antibody - middle region |
| Artikelnummer: | ASB-ARP55103_P050 |
| Hersteller Artikelnummer: | ARP55103_P050 |
| Alternativnummer: | ASB-ARP55103_P050-25UL,ASB-ARP55103_P050-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | WB |
| Spezies Reaktivität: | Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat |
| Immunogen: | The immunogen is a synthetic peptide directed towards the middle region of human CPEB3 |
| The exact function of CPEB3 remains unknown. |
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WB Suggested Anti-CPEB3 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: 721_B cell lysate |
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Host: Rabbit Target Name: CPEB3 Sample Type: 721_B Whole Cell Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1ug/ml Peptide Concentration: 5ug/ml Lysate Quantity: 25ug/lane Gel Concentration: 0.12% |
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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 0.5 ug/mL of the antibody was used in this experiment. The peptide is present in a ~52 kDa isoform. The protein may be modified by phosphorylation. |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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CPEB3 antibody - middle region (ARP55103_P050) in Human 721_B using Western Blot |
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