MED19 Antibody - middle region
Artikelnummer:
ASB-ARP55596_P050
- Bilder (5)
| Artikelname: | MED19 Antibody - middle region |
| Artikelnummer: | ASB-ARP55596_P050 |
| Hersteller Artikelnummer: | ARP55596_P050 |
| Alternativnummer: | ASB-ARP55596_P050-25UL,ASB-ARP55596_P050-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | ChIP, WB |
| Spezies Reaktivität: | Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish |
| Immunogen: | The immunogen is a synthetic peptide directed towards the middle region of Human MED19 |
| Alternative Synonym: | LCMR1, DT2P1G7, MED19AS |
| MED19 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II . |
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Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site. |
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Host: Rabbit Target Name: MED19 Sample Type: Jurkat Whole cell lysates Antibody Dilution: 1.0ug/ml |
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Host: Rabbit Target: MED19 Positive control (+): Rat testis (R-TE) Negative control (-): Rat kidney (R-KI) Antibody concentration: 1ug/ml |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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MED19 Antibody - middle region (ARP55596_P050) in Human Jurkat using Western Blot |
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