PRKACA Antibody - N-terminal region

Produktübersicht

• Artikelname: PRKACA Antibody - N-terminal region
• Artikelnummer: ASB-ARP57827_P050
• Hersteller Artikelnummer: ARP57827_P050
• Alternativnummer: ASB-ARP57827_P050-25UL,ASB-ARP57827_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: IHC, WB
• Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PRKACA
• Alternative Synonym: CAFD1, PKACA, PPNAD4

Produktbeschreibung

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 40 kDa
• NCBI: 5566
• UniProt: D4ACM4
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

Rabbit Anti-PRKACA antibody
Catalog Number: ARP57827
Formalin Fixed Paraffin Embedded Tissue: Human Adult Heart
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 a€'' 2.0 sec

Rabbit Anti-PRKACA Antibody
Catalog Number: ARP57827_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: N/A
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Rabbit Anti-PRKACA Antibody
Catalog Number: ARP57827_P050
Formalin Fixed Paraffin Embedded Tissue: Human Heart Muscle Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:600
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-PRKACA Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Placenta

Host: Rabbit
Target Name: PRKACA
Sample Tissue: Mouse Heart
Antibody Dilution: 1ug/ml

Host: Mouse
Target Name: PRKACA
Sample Tissue: Mouse Heart
Antibody Dilution: 1ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. The canonical isoform at 40 kDa is shown, and a smaller isoform of ~32 kDa also contains the peptide sequence, as do several other possible isoforms.

PRKACA antibody - N-terminal region (ARP57827_P050) in Human Placenta using Western Blot