H2AFY Antibody - N-terminal region

Produktübersicht

• Artikelname: H2AFY Antibody - N-terminal region
• Artikelnummer: ASB-ARP58284_P050
• Hersteller Artikelnummer: ARP58284_P050
• Alternativnummer: ASB-ARP58284_P050-25UL,ASB-ARP58284_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: WB
• Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human H2AFY
• Alternative Synonym: H2A.y, H2A/y, H2AFY, mH2A1, H2AF12M, MACROH2A1.1, macroH2A1.2

Produktbeschreibung

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. H2AFY is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 40 kDa
• NCBI: 9555
• UniProt: O75367
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

WB Suggested Anti-H2AFY Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: MCF7 cell lysate

Host: Rabbit
Target Name: H2AFY
Sample Type: MCF7
Antibody Dilution: 1.0ug/mlH2AFY is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. MacroH2A.1 has many splice isoforms ranging from ~18 kDa to the canonical isoform at 40 kDa. The immunizin

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Peptide is present in isoforms between 38 kDa and 40 kDa. Multiply modified by various post translational

H2AFY antibody - N-terminal region (ARP58284_P050) in Human MCF-7 using Western Blot