ACTN1 Antibody - N-terminal region
Artikelnummer:
ASB-ARP58413_P050
- Bilder (8)
| Artikelname: | ACTN1 Antibody - N-terminal region |
| Artikelnummer: | ASB-ARP58413_P050 |
| Hersteller Artikelnummer: | ARP58413_P050 |
| Alternativnummer: | ASB-ARP58413_P050-25UL,ASB-ARP58413_P050-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | IHC, WB |
| Spezies Reaktivität: | Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish |
| Immunogen: | The immunogen is a synthetic peptide directed towards the N terminal region of human ACTN1 |
| Alternative Synonym: | BDPLT15 |
| Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
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25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. |
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Sample Type: ACTNX-GFP transfected COS-7 cellsPrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-Alexa-Fluor 568Secondary Antibody Dilution: 1:100Color/Signal Descriptions: Green: GFP Red: ACTN1Gene Name: ACTN1Submitted by: Johannes W. Hell, Ph.D., Professor, Department of Pharmacology, University of California, Room 2419D Tupper Hall, Davis, CA 95616-5270, Phone: (530) 752 6540, Lab: (530) 752 7551, FAX: (530) 752 7710 |
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WB Suggested Anti-ACTN1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: 293T cell lysate |
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Sample Type: 1.-4. rACTN1-GFP + HEK293 (50ug) Primary Dilution: 1:1000-1:2000 Secondary Antibody: HRP conjugated goat anti-rabbit Secondary Dilution: 1:20,000 Image Submitted By: Magdalena Kalinowska Albert Einstein College of MedicineA,AA,A |
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ACTN1 antibody - N-terminal region (ARP58413_P050) validated by WB using 1.-2.Rat cortical neurons ctr siRNA (30ug) 2. Rat cortical neurons ACTN1 siRNA (30ug) at 1:1000-1:2000. |
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Lanes: Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:5000 Gene Name: ACTN1 Submitted by: Johannes W. Hell, University of California |
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Host: Rabbit Target Name: ACTN1 Sample Tissue: Human A549 Whole Cell Antibody Dilution: 1ug/ml |
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ACTN1 antibody - N-terminal region (ARP58413_P050) in Human 293T using Western Blot |
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