PDGFB Antibody - N-terminal region
Artikelnummer:
ASB-ARP58509_P050
- Bilder (8)
| Artikelname: | PDGFB Antibody - N-terminal region |
| Artikelnummer: | ASB-ARP58509_P050 |
| Hersteller Artikelnummer: | ARP58509_P050 |
| Alternativnummer: | ASB-ARP58509_P050-25UL,ASB-ARP58509_P050-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | IHC, WB |
| Spezies Reaktivität: | Bovine, Canine, Guinea pig, Human, Mouse, Rat, Sheep |
| Immunogen: | The immunogen is a synthetic peptide directed towards the N terminal region of human PDGFB |
| Alternative Synonym: | SIS, SSV, IBGC5, PDGF2, c-sis, PDGF-2 |
| PDGFB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor.The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene. |
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Immunohistochemistry with Uterus tissue at an antibody concentration of 5ug/ml using anti-PDGFB antibody (ARP58509_P050) |
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Rabbit Anti-PDGFB Antibody Catalog Number: ARP58509_P050 Formalin Fixed Paraffin Embedded Tissue: Human Adult liver Observed Staining: Cytoplasmic Primary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 a€'' 2.0 sec Protocol located in Reviews and Data. |
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WB Suggested Anti-PDGFB Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Positive Control: 293T cell lysateThere is BioGPS gene expression data showing that PDGFB is expressed in HEK293T |
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WB Suggested Anti-PDGFB antibody Titration: 1 ug/mL Sample Type: Human liver |
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Host: Rabbit Target Name: PDGFB Sample Tissue: Human MCF7 Whole Cell Antibody Dilution: 1ug/ml |
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Host: Rabbit Target Name: PDGFB Sample Tissue: Human A549 Whole Cell Antibody Dilution: 1ug/ml |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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PDGFB antibody - N-terminal region (ARP58509_P050) in Human 293T using Western Blot |
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