HEXA Antibody - C-terminal region

Produktübersicht

• Artikelname: HEXA Antibody - C-terminal region
• Artikelnummer: ASB-ARP61100_P050
• Hersteller Artikelnummer: ARP61100_P050
• Alternativnummer: ASB-ARP61100_P050-25UL,ASB-ARP61100_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: IHC, WB
• Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HEXA
• Alternative Synonym: TSD

Produktbeschreibung

This gene encodes the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Alpha subunit gene mutations lead to Tay-Sachs disease (GM2-gangliosidosis type I).

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 61 kDa
• NCBI: 3073
• UniProt: P06865
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 0.2 ug/mL of the antibody was used in this experiment. Protein is modified by glycosylation , and cleaved to a 50 kDa mature form.

Immunohistochemistry with Brain, cortex tissue at an antibody concentration of 5ug/ml using anti-HEXA antibody (ARP61100_P050)

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

WB Suggested Anti-HEXA Antibody Titration: 1 ug/ml
Positive Control: Fetal kidney lysate

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 0.5, 5, and 50 ug/mL. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for either two or three concentrations were averaged and results and standard deviation are shown.

HEXA antibody - C-terminal region (ARP61100_P050) in Human Fetal Kidney using Western Blot