Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits.
WB Suggested Anti-EIF6 Antibody Titration: 1.0 ug/ml Positive Control: Hela Whole CellEIF6 is supported by BioGPS gene expression data to be expressed in HeLa
Host: Rabbit Target Name: EIF6 Sample Type: Human 721_B Antibody Dilution: 1.0ug/mlEIF6 is supported by BioGPS gene expression data to be expressed in 721_B
Researcher: Rosa Carotenuto, Department of Structural and Functional Biology, University of Naples Federico II Application: Western blotting Species + Tissue/Cell type: Xenopus laevis embryos How many ugs of tissue/cell lysate run on the gel: 1. 50 ug xenopus laevis embryos lysate (lysate buffer) 2. 50 ug xenopus laevis embryos lysate (HB buffer) Primary antibody dilution: 1:500 Secondary antibody: Goat Anti-Rabbit AP Secondary antibody dilution: 1:20,000
EIF6 antibody - N-terminal region (ARP61388_P050) in Human HeLa using Western Blot
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