SPAM1 Antibody - C-terminal region

Produktübersicht

• Artikelname: SPAM1 Antibody - C-terminal region
• Artikelnummer: ASB-ARP64264_P050
• Hersteller Artikelnummer: ARP64264_P050
• Alternativnummer: ASB-ARP64264_P050-25UL,ASB-ARP64264_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: WB
• Spezies Reaktivität: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human SPAM1
• Alternative Synonym: HYA1, PH20, HYAL1, HYAL3, HYAL5, PH-20, SPAG15, HEL-S-96n

Produktbeschreibung

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1, another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 58kDa
• NCBI: 6677
• UniProt: P38567
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

WB Suggested Anti-SPAM1 Antibody
Titration: 1.0 ug/ml
Positive Control: Jurkat Whole Cell

Host: Rabbit
Target Name: SPAM1
Sample Type: 293T
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: SPAM1
Sample Type: Human Adult Placenta
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: SPAM1
Sample Tissue: Human HT1080 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: SPAM1
Sample Tissue: Human HepG2 Whole Cell
Antibody Dilution: 0.2ug/ml

Host: Rabbit
Target Name: SPAM1
Sample Tissue: Human A549 Whole Cell
Antibody Dilution: 3ug/ml

Host: Rabbit
Target Name: SPAM1
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 3ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

SPAM1 Antibody - C-terminal region (ARP64264_P050) in Human Jurkat using Western Blot