RPS28 Antibody - N-terminal region

Produktübersicht

• Artikelname: RPS28 Antibody - N-terminal region
• Artikelnummer: ASB-ARP65601_P050
• Hersteller Artikelnummer: ARP65601_P050
• Alternativnummer: ASB-ARP65601_P050-25UL,ASB-ARP65601_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: IHC, WB
• Spezies Reaktivität: Bovine, Equine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human RPS28
• Alternative Synonym: S28, eS28, DBA15

Produktbeschreibung

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S28E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 8kDa
• NCBI: 6234
• UniProt: P62857
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

Rabbit Anti-RPS28 Antibody
Catalog Number: ARP65601_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver
Observed Staining: Cytoplasm in Kupffer cells only, strong signal, very low tissue distribution
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 a€'' 2.0 sec
Protocol located in Reviews and Data.

WB Suggested Anti-RPS28 Antibody
Titration: 1.0 ug/ml
Positive Control: MDA-MB-435S Whole CellRPS28 is strongly supported by BioGPS gene expression data to be expressed in Human MDA-MB435 cells

Host: Rabbit
Target: RPS28
Positive control (+): THP-1 (N30)
Negative control (-): A549 (N03)
Antibody concentration: 3ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

RPS28 Antibody - N-terminal region (ARP65601_P050) in Human MDA-MB435 using Western Blot