PCNA Antibody - C-terminal region
Artikelnummer:
ASB-AVARP03018_T100
- Bilder (6)
| Artikelname: | PCNA Antibody - C-terminal region |
| Artikelnummer: | ASB-AVARP03018_T100 |
| Hersteller Artikelnummer: | AVARP03018_T100 |
| Alternativnummer: | ASB-AVARP03018_T100-25UL,ASB-AVARP03018_T100-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | IHC, WB |
| Spezies Reaktivität: | Bovine, Canine, Human, Mouse, Rat, Zebrafish |
| Immunogen: | The immunogen is a synthetic peptide directed towards the C terminal region of human PCNA |
| Alternative Synonym: | ATLD2 |
| PCNA is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerases processibility during elongation of the leading strand.The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
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Data Provided by Dr. Yuzhi Chen, University of Arkansas for Medical SciencesNT2 cells were pretreated with 2N HCL for 30 minutesWashed 3x in PBSStained for 2 hours with 1ug/50ul antibodyand Incubated in Alexa goat-rabbit 594 for 1H.Images were obtained with a 40x objectiveNT-2 cellsAntibody: RedDAPI: Blue |
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Sample Type: 1. Human NT-2 cells (60ug) Primary antibody dilution: 2ug/ml Secondary antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary antibody dilution: 1: 20,000 Image Submitted by: Yuzhi ChenUniversity of Arkansas for Medical Science |
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Rabbit Anti-PCNA Antibody Catalog Number: AVARP03018 Paraffin Embedded Tissue: Human Spleen Cellular Data: Spleen cells Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X |
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Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science Application: IHC Species+tissue/cell type: Human brain stem cells Primary antibody dilution: 1:500 Secondary antibody: Goat anti-rabbit Alexa-Fluor 594 Secondary antibody dilution: 1:1000 |
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WB Suggested Anti-PCNA Antibody Titration: 1.25ug/ml Positive Control: HepG2 cell lysatePCNA is supported by BioGPS gene expression data to be expressed in HepG2 |
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PCNA antibody - C-terminal region (AVARP03018_T100) in Human NT-2 using Western Blot |
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