Mitochondrial Permeability Transition Pore Assay Kit s a more direct detection method for detecting the opening of MPTP than only based on mitochondrial membrane potential analysis
Tag:
Mitochondrial Permeability Transition
Anwendungsbeschreibung:
Mitochondrial Permeability Transition Pore Assay Kit is a more direct detection method for detecting the opening of MPTP than only based on mitochondrial membrane potential analysis. The principle is: Firstly, loading Calcein AM through passive transportation, which is a kind of cell staining reagent for fluorescent labeling of living cells. It can easily penetrate the living cell membrane and is cleaved by intracellular esterase to form the membrane-impermeable polar molecule Calcein, which is then trapped in the cell and causes the cytoplasm including mitochondria to emit strong green fluorescence. After adding CoCl2, the fluorescence from the cytoplasm is quenched by CoCl2, only left fluorescence in the mitochondria. As a control, cells can be loaded with Calcein AM and CoCl2, and treated with Ionomycin, so as to make cells load more Ca2+, which causes the activation of MPTP and quenching of mitochondrial fluorescence.
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