Rat Nuclear factor-kappa B (NF-kB) ELISA Kit

Artikelnummer: ASC-KTE100827
Artikelname: Rat Nuclear factor-kappa B (NF-kB) ELISA Kit
Artikelnummer: ASC-KTE100827
Hersteller Artikelnummer: KTE100827
Alternativnummer: ASC-KTE100827-48,ASC-KTE100827-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Rat
Alternative Synonym: NFKB1, DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NF-kappaB, NFKB-p105, NFKB-p50, p105, p50, DNA binding factor KBF1, NF-kappabeta, nuclear factor NF-kappa-B p50 subunit, nuclear factor kappa-B DNA bi
This Rat Nuclear factor-kappa B (NF-kB) ELISA Kit employs a two-site sandwich ELISA to quantitate NFKB1.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: NFKB1
UniProt: Q63369
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Rat Nuclear factor-kappa B (NF-kB) ELISA Kit employs a two-site sandwich ELISA to quantitate NFKB1 in samples. An antibody specific for NFKB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNFKB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NFKB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NFKB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.