This Rat Estradiol antibody (E2-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E2-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated E2-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled E2-Ab and unlabeled E2-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of E2-Ab in the sample. The color development is stopped and the intensity of the color is measured.
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