This Human Pantetheinase (VNN1) ELISA Kit employs a two-site sandwich ELISA to quantitate VNN1 in samples. An antibody specific for VNN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVNN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VNN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VNN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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