Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit

Artikelnummer: ASC-KTE60098
Artikelname: Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit
Artikelnummer: ASC-KTE60098
Hersteller Artikelnummer: KTE60098
Alternativnummer: ASC-KTE60098-48, ASC-KTE60098-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: UBE3A, ANCR, AS, E6-AP, EPVE6AP, FLJ26981, HPVE6A, CTCL tumor antigen se37-2, human papilloma virus E6-associated protein, oncogenic protein-associated protein E6-AP
This Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE3A.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: UBE3A
NCBI: 7337
UniProt: Q05086
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE3A in samples. An antibody specific for UBE3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE3A bound in the initial step. The color development is stopped and the intensity of the color is measured.