Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit

Artikelnummer: ASC-KTE60102
Artikelname: Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit
Artikelnummer: ASC-KTE60102
Hersteller Artikelnummer: KTE60102
Alternativnummer: ASC-KTE60102-48, ASC-KTE60102-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: UBE2C, RP3-447F3.1, UBCH10, dJ447F3.2, cyclin-selective ubiquitin carrier protein, mitotic-specific ubiquitin-conjugating enzyme, ubiquitin carrier protein E2-C, ubiquitin-protein ligase C
This Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2C.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: UBE2C
NCBI: 11065
UniProt: O00762
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Ubiquitin-conjugating enzyme E2 C (UBE2C) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2C in samples. An antibody specific for UBE2C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE2C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2C bound in the initial step. The color development is stopped and the intensity of the color is measured.