EliKine(TM) Human Leptin ELISA Kit

Artikelnummer: ASC-KTE6026
Artikelname: EliKine(TM) Human Leptin ELISA Kit
Artikelnummer: ASC-KTE6026
Hersteller Artikelnummer: KTE6026
Alternativnummer: ASC-KTE6026-48, ASC-KTE6026-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: FLJ94114, LEP, LEP_HUMAN, LEPD, Leptin, Leptin Precursor Obesity Factor, OB, Obese protein
EliKine(TM) Human Leptin ELISA Kit employs a two-site sandwich ELISA to quantitate Human Leptin .
Detektionsbereich: 31.25 pg/mL- 2000 pg/mL
Sensitivitaet: 16 pg/mL
Tag: LEP
NCBI: 3952
UniProt: P41159
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: EliKine(TM) Human Leptin ELISA Kit employs a two-site sandwich ELISA to quantitate Leptin in samples. An antibody specific for Leptin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Leptin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Leptin is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound in the initial step. The color development is stopped and the intensity of the color is measured.