Human Tubulointerstitial nephritis antigen-like (TINAGL1) ELISA Kit employs a two-site sandwich ELISA to quantitate TINAGL1 in samples. An antibody specific for TINAGL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TINAGL1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human TINAGL1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of TINAGL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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