EliKine(TM) Human VEGF ELISA Kit

Artikelnummer: ASC-KTE6033
Artikelname: EliKine(TM) Human VEGF ELISA Kit
Artikelnummer: ASC-KTE6033
Hersteller Artikelnummer: KTE6033
Alternativnummer: ASC-KTE6033-48, ASC-KTE6033-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: VEGFA, RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165, vascular permeability factor
EliKine(TM) Human VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate Human VEGF-A.
Detektionsbereich: 31.25 pg/mL-2000 pg/mL
Sensitivitaet: 16 pg/mL
Tag: VEGFA
NCBI: 7422
UniProt: P15692
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: EliKine(TM) Human VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF-A in samples. An antibody specific for VEGF-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF-A is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF-A bound in the initial step. The color development is stopped and the intensity of the color is measured.