EliKine(TM) Human Alpha Fetoprotein (AFP) ELISA Kit

Produktübersicht

• Artikelname: EliKine(TM) Human Alpha Fetoprotein (AFP) ELISA Kit
• Artikelnummer: ASC-KTE6037
• Hersteller Artikelnummer: KTE6037
• Alternativnummer: ASC-KTE6037-48, ASC-KTE6037-96
• Hersteller: Abbkine Scientific
• Kategorie: Kits/Assays
• Applikation: ELISA
• Spezies Reaktivität: Human

Produktbeschreibung

EliKine(TM) Human Alpha Fetoprotein (AFP) ELISA Kit employs a two-site sandwich ELISA to quantitate Human AFP.

Produkteigenschaften

• Detektionsbereich: 0.625-40 ng/mL
• Sensitivitaet: 0.313 ng/mL
• Tag: AFP
• Proben: SerumPlasma Cell culture supernatants

Anwendungsbeschreibung

• Anwendungsbeschreibung: EliKine(TM) Human Alpha Fetoprotein (AFP) ELISA Kit employs a double antibody sandwich method to quantitate Human AFP in samples. An antibody specific for Human AFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human AFP present is bound by the immobilized antibody. After removing any unbound substances, an HRP-conjugated antibody specific for Human AFP is added to the wells. After washing, remove any unbound HRP-conjugated antibody reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Human Alpha Fetoprotein (AFP) concentration is proportional to the OD450 nm value.