This Human Taste receptor type 2 member 42 (TAS2R42) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS2R42 in samples. An antibody specific for TAS2R42 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAS2R42 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAS2R42 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAS2R42 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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