Human Mothers against decapentaplegic homolog 4 (SMAD4) ELISA Kit

Artikelnummer: ASC-KTE60573
Artikelname: Human Mothers against decapentaplegic homolog 4 (SMAD4) ELISA Kit
Artikelnummer: ASC-KTE60573
Hersteller Artikelnummer: KTE60573
Alternativnummer: ASC-KTE60573-48, ASC-KTE60573-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: SMAD4, DPC4, JIP, MADH4, MAD, mothers against decapentaplegic homolog 4, OTTHUMP00000163548, SMAD, mothers against DPP homolog 4, deleted in pancreatic carcinoma locus 4, mothers against decapentaplegic homolo
This Human Mothers against decapentaplegic homolog 4 (SMAD4) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD4.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: SMAD4
NCBI: 4089
UniProt: Q13485
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Mothers against decapentaplegic homolog 4 (SMAD4) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD4 in samples. An antibody specific for SMAD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMAD4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.