Human Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA) ELISA Kit

Artikelnummer: ASC-KTE60657
Artikelname: Human Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA) ELISA Kit
Artikelnummer: ASC-KTE60657
Hersteller Artikelnummer: KTE60657
Alternativnummer: ASC-KTE60657-48, ASC-KTE60657-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: SIRPA, BIT, CD172A, MFR, MYD-1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2, SHP substrate-1, brain-immunoglobulin-like molecule with tyrosine-based activation motifs, macrophage fu
This Human Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRPA.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: SIRPA
NCBI: 140885
UniProt: P78324
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRPA in samples. An antibody specific for SIRPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIRPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRPA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRPA bound in the initial step. The color development is stopped and the intensity of the color is measured.