Human Visceral adipose-specific serine protease inhibitor (vaspin) ELISA Kit employs a two-site sandwich ELISA to quantitate vaspin in samples. An antibody specific for vaspin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any vaspin present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human vaspin detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of vaspin bound in the initial step. The color development is stopped and the intensity of the color is measured.
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