Human Semaphorin 3F (S3F) ELISA Kit

Artikelnummer: ASC-KTE60714
Artikelname: Human Semaphorin 3F (S3F) ELISA Kit
Artikelnummer: ASC-KTE60714
Hersteller Artikelnummer: KTE60714
Alternativnummer: ASC-KTE60714-48, ASC-KTE60714-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: SEMA3F, SEMA-IV, SEMA4, SEMAK, sema IV, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F, semaphorin 3F, semaphorinI/F, semaphorin IV
This Human Semaphorin 3F (S3F) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3F.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: SEMA3F
NCBI: 6405
UniProt: Q13275
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Semaphorin 3F (S3F) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3F in samples. An antibody specific for SEMA3F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA3F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3F is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3F bound in the initial step. The color development is stopped and the intensity of the color is measured.