Human Semaphorin 3A (SEMA3A) ELISA Kit

Artikelnummer: ASC-KTE60717
Artikelname: Human Semaphorin 3A (SEMA3A) ELISA Kit
Artikelnummer: ASC-KTE60717
Hersteller Artikelnummer: KTE60717
Alternativnummer: ASC-KTE60717-48, ASC-KTE60717-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: SEMA3A, Hsema-I, Hsema-III, MGC133243, SEMA1, SEMAD, SEMAIII, SEMAL, SemD, coll-1, OTTHUMP00000208884, collapsin 1, semaphorin 3A, semaphorin D, semaphorinI, semaphorin L, semaphorin-like
This Human Semaphorin 3A (SEMA3A) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3A.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: SEMA3A
NCBI: 10371
UniProt: Q14563
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Semaphorin 3A (SEMA3A) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3A in samples. An antibody specific for SEMA3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3A bound in the initial step. The color development is stopped and the intensity of the color is measured.