Human Sphingosine 1-phosphate receptor type 1 (S1P1) ELISA Kit

Artikelnummer: ASC-KTE60756
Artikelname: Human Sphingosine 1-phosphate receptor type 1 (S1P1) ELISA Kit
Artikelnummer: ASC-KTE60756
Hersteller Artikelnummer: KTE60756
Alternativnummer: ASC-KTE60756-48, ASC-KTE60756-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: S1PR1, CHEDG1, D1S3362, ECGF1, EDG-1, EDG1, FLJ58121, S1P1, endothelial differentiation, sphingolipid G-protein-coupled receptor, 1, sphingosine 1-phosphate receptor EDG1
This Human Sphingosine 1-phosphate receptor type 1 (S1P1) ELISA Kit employs a two-site sandwich ELISA to quantitate S1PR1.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: S1PR1
NCBI: 1901
UniProt: P21453
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Sphingosine 1-phosphate receptor type 1 (S1P1) ELISA Kit employs a two-site sandwich ELISA to quantitate S1PR1 in samples. An antibody specific for S1PR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS1PR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S1PR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S1PR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.