Human Nuclear factor-KB p65 (NF-KB p65) ELISA Kit

Artikelnummer: ASC-KTE60811
Artikelname: Human Nuclear factor-KB p65 (NF-KB p65) ELISA Kit
Artikelnummer: ASC-KTE60811
Hersteller Artikelnummer: KTE60811
Alternativnummer: ASC-KTE60811-48, ASC-KTE60811-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: RELA, MGC131774, NFKB3, p65, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene
This Human Nuclear factor-KB p65 (NF-KB p65) ELISA Kit employs a two-site sandwich ELISA to quantitate RELA.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: RELA
NCBI: 5970
UniProt: Q04206
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Nuclear factor-KB p65 (NF-KB p65) ELISA Kit employs a two-site sandwich ELISA to quantitate RELA in samples. An antibody specific for RELA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRELA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RELA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RELA bound in the initial step. The color development is stopped and the intensity of the color is measured.