Human 5-Hydroxytryptamine (5-HT) ELISA Kit employs a two-site sandwich ELISA to quantitate 5-HT in samples. An antibody specific for 5-HT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 5-HT present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human 5-HT detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of 5-HT bound in the initial step. The color development is stopped and the intensity of the color is measured.
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