Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit

Artikelnummer: ASC-KTE60910
Artikelname: Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit
Artikelnummer: ASC-KTE60910
Hersteller Artikelnummer: KTE60910
Alternativnummer: ASC-KTE60910-48, ASC-KTE60910-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: ADAM12, RP11-295J3.5, MCMP, MCMPMltna, MLTN, MLTNA, a disintegrin and metalloproteinase domain 12 (meltrin alpha), meltrin alpha
This Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM12.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: ADAM12
NCBI: 8038
UniProt: O43184
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM12 in samples. An antibody specific for ADAM12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM12 bound in the initial step. The color development is stopped and the intensity of the color is measured.