Human TNF alpha converting enzyme (TACE) ELISA Kit

Artikelnummer: ASC-KTE60912
Artikelname: Human TNF alpha converting enzyme (TACE) ELISA Kit
Artikelnummer: ASC-KTE60912
Hersteller Artikelnummer: KTE60912
Alternativnummer: ASC-KTE60912-48, ASC-KTE60912-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: ADAM17, ADAM18, CD156B, CSVP, MGC71942, TACE, ADAM metallopeptidase domain 18, TNF-alpha converting enzyme, a disintegrin and metalloprotease domain 17, snake venom-like protease, tumor necrosis factor, alpha,
This Human TNF alpha converting enzyme (TACE) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM17.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: ADAM17
NCBI: 6868
UniProt: P78536
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human TNF alpha converting enzyme (TACE) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM17 in samples. An antibody specific for ADAM17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM17 bound in the initial step. The color development is stopped and the intensity of the color is measured.