Human Alcohol dehydrogenase (ADH) ELISA Kit

Artikelnummer: ASC-KTE60935
Artikelname: Human Alcohol dehydrogenase (ADH) ELISA Kit
Artikelnummer: ASC-KTE60935
Hersteller Artikelnummer: KTE60935
Alternativnummer: ASC-KTE60935-48, ASC-KTE60935-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: ADH1A, ADH1, ADH, alpha subunit, alcohol dehydrogenase 1 (class I), alpha polypeptide, aldehyde reductase, class I alcohol dehydrogenase, alpha subunit
This Human Alcohol dehydrogenase (ADH) ELISA Kit employs a two-site sandwich ELISA to quantitate ADH1A.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: ADH1A
NCBI: 124
UniProt: P07327
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Alcohol dehydrogenase (ADH) ELISA Kit employs a two-site sandwich ELISA to quantitate ADH1A in samples. An antibody specific for ADH1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADH1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADH1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADH1A bound in the initial step. The color development is stopped and the intensity of the color is measured.