Human Advanced glycation end products (AGEs) ELISA Kit employs a two-site sandwich ELISA to quantitate AGEs in samples. An antibody specific for AGEs has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGEs present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human AGEs detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of AGEs bound in the initial step. The color development is stopped and the intensity of the color is measured.
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