Human Rap guanine nucleotide exchange factor 4 (RAPGEF4) ELISA Kit

Artikelnummer: ASC-KTE60978
Artikelname: Human Rap guanine nucleotide exchange factor 4 (RAPGEF4) ELISA Kit
Artikelnummer: ASC-KTE60978
Hersteller Artikelnummer: KTE60978
Alternativnummer: ASC-KTE60978-48, ASC-KTE60978-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: RAPGEF4, CAMP-GEFII, CGEF2, EPAC2, Nbla00496, RAP guanine-nucleotide-exchange factor (GEF) 4, cAMP-regulated guanine nucleotide exchange factor, exchange protein directly activated by cAMP 2, putative protei
This Human Rap guanine nucleotide exchange factor 4 (RAPGEF4) ELISA Kit employs a two-site sandwich ELISA to quantitate RAPGEF4.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: RAPGEF4
NCBI: 11069
UniProt: Q8WZA2
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Rap guanine nucleotide exchange factor 4 (RAPGEF4) ELISA Kit employs a two-site sandwich ELISA to quantitate RAPGEF4 in samples. An antibody specific for RAPGEF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAPGEF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAPGEF4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAPGEF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.