Human Phosphatase and tension homolog deleted on chromosome ten (PTEN/MMAC1) ELISA Kit

Produktübersicht

• Artikelname: Human Phosphatase and tension homolog deleted on chromosome ten (PTEN/MMAC1) ELISA Kit
• Artikelnummer: ASC-KTE61074
• Hersteller Artikelnummer: KTE61074
• Alternativnummer: ASC-KTE61074-48, ASC-KTE61074-96
• Hersteller: Abbkine Scientific
• Kategorie: Kits/Assays
• Applikation: ELISA
• Spezies Reaktivität: Human
• Alternative Synonym: PTEN, 10q23del, BZS, MGC11227, MHAM, MMAC1, PTEN1, TEP1, MMAC1 phosphatase and tensin homolog deleted on chromosome 10, mutated in multiple advanced cancers 1

Produktbeschreibung

This Human Phosphatase and tension homolog deleted on chromosome ten (PTEN/MMAC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTEN.

Produkteigenschaften

• Detektionsbereich: Please inquire
• Sensitivitaet: Please inquire
• Tag: PTEN
• NCBI: 5728
• UniProt: P60484
• Proben: Cell culture supernatantsSerumPlasmaOther biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: This Human Phosphatase and tension homolog deleted on chromosome ten (PTEN/MMAC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTEN in samples. An antibody specific for PTEN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTEN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTEN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTEN bound in the initial step. The color development is stopped and the intensity of the color is measured.