This Human Nucleolin (NCL) ELISA Kit employs a two-site sandwich ELISA to quantitate NCL in samples. An antibody specific for NCL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNCL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCL bound in the initial step. The color development is stopped and the intensity of the color is measured.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
