Human Mediator of RNA polymerase II transcription subunit 20 (MED20) ELISA Kit

Artikelnummer: ASC-KTE61663
Artikelname: Human Mediator of RNA polymerase II transcription subunit 20 (MED20) ELISA Kit
Artikelnummer: ASC-KTE61663
Hersteller Artikelnummer: KTE61663
Alternativnummer: ASC-KTE61663-48, ASC-KTE61663-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: MED20, RP5-973N23.1, DKFZp586D2223, MGC29869, PRO0213, TRFP, Trf (TATA binding protein-related factor)-proximal homolog
This Human Mediator of RNA polymerase II transcription subunit 20 (MED20) ELISA Kit employs a two-site sandwich ELISA to quantitate MED20.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: MED20
NCBI: 9477
UniProt: Q9H944
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Mediator of RNA polymerase II transcription subunit 20 (MED20) ELISA Kit employs a two-site sandwich ELISA to quantitate MED20 in samples. An antibody specific for MED20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED20 bound in the initial step. The color development is stopped and the intensity of the color is measured.