Human Mediator of RNA polymerase II transcription subunit 16 (MED16) ELISA Kit

Artikelnummer: ASC-KTE61666
Artikelname: Human Mediator of RNA polymerase II transcription subunit 16 (MED16) ELISA Kit
Artikelnummer: ASC-KTE61666
Hersteller Artikelnummer: KTE61666
Alternativnummer: ASC-KTE61666-48, ASC-KTE61666-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: MED16, DRIP92, THRAP5, TRAP95, thyroid hormone receptor-associated protein 5, thyroid hormone receptor-associated protein, 95-kD subunit
This Human Mediator of RNA polymerase II transcription subunit 16 (MED16) ELISA Kit employs a two-site sandwich ELISA to quantitate MED16.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: MED16
NCBI: 10025
UniProt: Q9Y2X0
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Mediator of RNA polymerase II transcription subunit 16 (MED16) ELISA Kit employs a two-site sandwich ELISA to quantitate MED16 in samples. An antibody specific for MED16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED16 bound in the initial step. The color development is stopped and the intensity of the color is measured.