Human Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit

Artikelnummer: ASC-KTE61667
Artikelname: Human Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit
Artikelnummer: ASC-KTE61667
Hersteller Artikelnummer: KTE61667
Alternativnummer: ASC-KTE61667-48, ASC-KTE61667-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: MED14, CRSP150, CRSP2, CSRP, CXorf4, DRIP150, EXLM1, MGC104513, RGR1, TRAP170, cofactor required for Sp1 transcriptional activation, subunit 2 (150kD), cofactor required for Sp1 transcriptional activation,
This Human Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit employs a two-site sandwich ELISA to quantitate MED14.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: MED14
NCBI: 9282
UniProt: O60244
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Mediator of RNA polymerase II transcription subunit 14 (MED14) ELISA Kit employs a two-site sandwich ELISA to quantitate MED14 in samples. An antibody specific for MED14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED14 bound in the initial step. The color development is stopped and the intensity of the color is measured.