Human Mediator of RNA polymerase II transcription subunit 12-like protein (MED12L) ELISA Kit

Artikelnummer: ASC-KTE61670
Artikelname: Human Mediator of RNA polymerase II transcription subunit 12-like protein (MED12L) ELISA Kit
Artikelnummer: ASC-KTE61670
Hersteller Artikelnummer: KTE61670
Alternativnummer: ASC-KTE61670-48, ASC-KTE61670-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: MED12L, FLJ46288, KIAA1635, NOPAR, TNRC11L, TRALP, TRALPUSH, mediator of RNA polymerase transcription, subunit 12 homolog (S. cerevisiae)-like, mediator of RNA polymerase transcription, subunit 12 homo
This Human Mediator of RNA polymerase II transcription subunit 12-like protein (MED12L) ELISA Kit employs a two-site sandwich ELISA to quantitate MED12L.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: MED12L
NCBI: 116931
UniProt: Q86YW9
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Mediator of RNA polymerase II transcription subunit 12-like protein (MED12L) ELISA Kit employs a two-site sandwich ELISA to quantitate MED12L in samples. An antibody specific for MED12L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED12L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED12L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED12L bound in the initial step. The color development is stopped and the intensity of the color is measured.