Human Mannan-binding lectin serine peptidase 2 (MASP2) ELISA Kit employs a two-site sandwich ELISA to quantitate MASP2 in samples. An antibody specific for MASP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MASP2 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human MASP2 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MASP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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